Scans surfaces with laser and detects fluorescence

Typical applications: imaging of gels

RT PCR experiments

Typical applications: quantification of DNA, time resolved analysis

Device to measure equilibrium constants and reaction rates of bio macromolecules

Typical applications: binding rates and affinities of all kinds of biomolecular interactors

Soft lithography is done in the cleanroom (ZNN, ground floor) equipped with : wet benches, fume hoods, spin coater, standard thin film materials, critical point dryer and optical lithography with maskless aligner; The microfluidic profile of the wafers is checked with DekTak surface profiler. Microfluidic devices are further fabricated by polymer hardening and plasma bonding (Oxigen Plasma cleaner with vacuum pump) ZNN, second floor 2.010. As a quality control, the microfluidic devices are checked with microfluidic flow controllers (pressure pumps incl. syringe pumps, valve distribution, flow sensors and costumized equipment such as holders and electronics) on a  IX81 microscope

Typical application: gene expression studies in single cell, emulsion droplets, cell extract

The pool comprises the following devices:

• Jasco UV/Vis spectrometer for absorption and transmission measurements
• Jasco CD and UV/Vis spectrometer for circular dichroism, absorption and transmission measurements with temperature control
• Cary Ecliples fluorescence spectrometer with four sample holder for measurements of fluorescence emission, fluorescence absorption and emission spectra with temperature control
• Fluorolog-3 fluorescence spectrometer for measurements of fluorescence emission
• Wyatt dynamic light scattering for measurements of particle sizes in aqueous solution

Typical applications: spectrometric studies on biochemical and biological samples

The Partec CyFlow Cube is a fully equipped desktop Flow Cytometer (FCM). The instrument features a modular optical concept and allows the usage of different lasers as light source and the detection of up to 8 optical channnels (3 colours + FSC + SSC when sorter is installed). Currently the flow cytometer is equipped with a blue 488nm and a green 532nm laser. Cube 8 runs with an internal PC. Data acquisition, instrument control, and data analysis are controlled and performed with CyView software. The cell sorter module is a optional add-on for the Cube 8 and combines a flow chamber with a piezo element, including electric activation with the goal to sort cells or particles non-destructively.

Typical application: gene expression studies in bacteria and eukaryotic cells